Fig. 6
Suppression of human T-cell leukemia progress in xenograft models by CRO-CD7 CAR-iNK cells. A Schematic diagram of in vivo studies with luciferase-expressing CCRF-CEM cells (CCRF-CEM-luci) in mouse xenograft models. The CCRF-CEM-luci tumor cells were injected into B-NDG hIL15 mice (1 × 105/mouse) via the tail vein on day 0. The mice were irradiated (1.0 Gy) on day 1. Equal numbers (1 × 107) of Ctrl-iNK, CD7 KO-CD7 CAR-iNK, or CRO-CD7 CAR-iNK cells were injected into each animal on day 1, day 4, and day 7. BLI was performed every week. B BLI images of the xenograft models at the indicated time points (Tumor + PBS, Tumor + Ctrl-iNK, Tumor + CD7 KO-CD7 CAR-iNK, and Tumor + CRO-CD7 CAR-iNK, n = 5 mice in each group). The radiance indicates tumor burden. C Total flux (p/s) of the xenograft models measured at the indicated time points (n = 5 mice in each group). Two-tailed independent t-test was used for statistics. NS, not significant (p > 0.05), *p < 0.05, **p < 0.01, ***p < 0.001. D Kaplan-Meier survival curves of the xenograft models (n = 5 mice in each group). Median survival: Tumor + PBS, 29 days; Tumor + Ctrl-iNK, 30 days; Tumor + CD7 KO-CD7 CAR-iNK, 32 days; Tumor + CRO-CD7 CAR-iNK, 36 days. The logarithmic rank (Mantel-Cox) test was used for statistics. NS, not significant, *p < 0.05, **p < 0.01