Fig. 5
Dynamic analysis of CRO-CD7 CAR-iNK cells in the B-NDG hIL15 mice after injection. A Strategy for the construction of CXCR4-CD7 KO-CD7 CAR-hPSCs (CRO-CD7 CAR-hPSCs), CD7 KO-CD7 CAR-luciferase-hPSCs (CD7 KO-CD7 CAR-luci-hPSCs), and CXCR4-CD7 KO-CD7 CAR-luciferase-hPSCs (CRO-CD7 CAR-luci-hPSCs). B BLI images of the CD7 KO-CD7 CAR-luci-hPSCs and CRO-CD7 CAR-luci-hPSCs. C Representative flow cytometry plots of CRO-CD7 CAR-iNK, CD7 KO-CD7 CAR-luci-iNK, and CRO-CD7 CAR-luci-iNK cells on day 27. D Schematic diagram of in vivo studies with CD7 KO-CD7 CAR-luci-iNK and CRO-CD7 CAR-luci-iNK cells in B-NDG hIL15 mice. The CD7 KO-CD7 CAR-luci-iNK and CRO-CD7 CAR-luci-iNK cells were injected into B-NDG hIL15 mice (1 × 107 cells/mouse) via the tail vein on day 0, respectively. BLI and flow cytometry analysis were performed at the indicated time points. E BLI images showing the presence of CD7 KO-CD7 CAR-luci-iNK or CRO-CD7 CAR-luci-iNK cells in B-NDG hIL15 mice (n = 2 mice in each group) at the indicated time points. F Total flux (p/s) of the CD7 KO-CD7 CAR-luci-iNK or CRO-CD7 CAR-luci-iNK cells injected B-NDG hIL15 mice measured at the indicated time points (n = 2 mice in each group). G Flow cytometry analysis of CD7 KO-CD7 CAR-luci-iNK or CRO-CD7 CAR-luci-iNK cells in the PB of B-NDG hIL15 mice at the indicated time points