Your privacy, your choice

We use essential cookies to make sure the site can function. We also use optional cookies for advertising, personalisation of content, usage analysis, and social media.

By accepting optional cookies, you consent to the processing of your personal data - including transfers to third parties. Some third parties are outside of the European Economic Area, with varying standards of data protection.

See our privacy policy for more information on the use of your personal data.

for further information and to change your choices.

You are viewing the site in preview mode

Skip to main content
Fig. 3 | Journal of Hematology & Oncology

Fig. 3

From: Engineered CRO-CD7 CAR-NK cells derived from pluripotent stem cells avoid fratricide and efficiently suppress human T-cell malignancies

Fig. 3

The cytotoxic activity of CD7 KO-CD7 CAR-iNK cells against CD7+ tumor cell lines in vitro. A Experimental design for the tumor-killing assay. The Ctrl-iNK and CD7 KO-CD7 CAR-iNK cells (effectors, E) were cocultured with Jurkat or CCRF-CEM tumor cells labeled with eFluor™ 670 (targets, T), respectively. The E:T ratios include 0.05:1, 0.1:1, 0.2:1, 0.4:1, 0.8:1, 1.6:1, 3.2:1, and 5:1. B-C Cytotoxicity analysis of Ctrl-iNK cells and CD7 KO-CD7 CAR-iNK cells against Jurkat (B) or CCRF-CEM (C) tumor cells at the indicated E:T ratios after 4-h incubation. Data are represented as mean ± SD (n = 4). Mann-Whitney U test was used for statistics. ***p < 0.001. D Experimental design for the multiple rounds of tumor killing. The Ctrl-iNK and CD7 KO-CD7 CAR-iNK cells were respectively cocultured with Jurkat or CCRF-CEM tumor cells for 12 h per round at the E:T ratio of 1:1. Fresh tumor cells were added to the Ctrl-iNK/CD7 KO-CD7 CAR-iNK cell residues incubated every other 12 h. E Cytotoxicity analysis of three consecutive rounds of Jurkat cell killing by Ctrl-iNK or CD7 KO-CD7 CAR-iNK cells. Data are represented as mean ± SD (n = 4). Two-tailed independent t-test was used for statistics. ***p < 0.001. F Cytotoxicity analysis of three consecutive rounds of CCRF-CEM cell killing by Ctrl-iNK or CD7 KO-CD7 CAR-iNK cells. Data are represented as mean ± SD (n = 4). Two-tailed independent t-test was used for statistics. ***p < 0.001. G-H Assessment of CD107a expression by Ctrl-iNK or CD7 KO-CD7 CAR-iNK cells following 4 h of coculture with Jurkat/CCRF-CEM tumor cells at the ratio of 1:1. Data are represented as mean ± SD (n = 3). Two-tailed independent t-test was used for statistics. **p < 0.01, ***p < 0.001. I-J Measurement of IFN-γ production by Ctrl-iNK or CD7 KO-CD7 CAR-iNK cells in response to Jurkat/CCRF-CEM tumor cells. The Ctrl-iNK or CD7 KO-CD7 CAR-iNK cells were stimulated by Jurkat/CCRF-CEM at the ratio of 1:1 for 4 h. Data are represented as mean ± SD (n = 3). Two-tailed independent t-test was used for statistics. NS, not significant (p > 0.05), ***p < 0.001. K-L Evaluation of TNF-α production by Ctrl-iNK or CD7 KO-CD7 CAR-iNK cells in response to Jurkat/CCRF-CEM tumor cells. The Ctrl-iNK or CD7 KO-CD7 CAR-iNK cells were stimulated by Jurkat/CCRF-CEM at the ratio of 1:1 for 4 h. Data are represented as mean ± SD (n = 3). Two-tailed independent t-test was used for statistics. NS, not significant (p > 0.05), **p < 0.01

Back to article page

Journal of Hematology & Oncology

ISSN: 1756-8722

Contact us