Fig. 3
MK C3G regulates the mobilization of progenitors following 5-FU treatment (A, B) BM cellularity is depicted as the mean ± SEM of cells from one femur and one tibia of (A) wtC3GPf4 and tgC3GPf4 or (B) C3GPf4-wt and C3GPf4-KO mice throughout the recovery after 5-FU injection. Box plots depict the median and range of (C, L) LKS, (D, M) LKS−, (E, N) LT-HSC, (F, O) ST-HSC, (G, P) MPP, (H, Q) CLP, (I, R) CMP, (J, S) GMP, (K, T) MEP and (Y, Z) Lin− subpopulations of HSPCs in the BM of tgC3GPf4 or C3GPf4-KO mice (as indicated) compared to their corresponding wild-type littermates 7 days after 5-FU administration. The values correspond to the proportion within each corresponding parent gate and are normalized to the corresponding wild-types. (U, V) Percentage of Lin− cells in BM (mean ± SEM) from (U) wtC3GPf4 and tgC3GPf4 or (V) C3GPf4-wt and C3GPf4-KO mice during the 21-day assessment period. (W, X) Box plots depict the median value and range of the BrdU+ Lin− cells from (W) tgC3GPf4 mice and (X) C3GPf4-KO mice normalized against their controls, 7 days after the injection of 5-FU. Two independent experiments were performed. Statistics were performed using two-way ANOVA with uncorrected Fisher’s LSD post-hoc test in (A, B, U, V), and using t-test in (C–T, W–Z). *p ≤ 0.05. **p ≤ 0.01. ***p ≤ 0.001. Three independent experiments were performed